AbstractMacrophage behavior upon biomaterial implantation conditions the inflammatory
response, and the regenerative process. Modulating macrophage phenotype from M1 to M2 is an
increasingly explored strategy in biomaterial development. Previous work from our group, showed
that Fibrinogen (Fg) interacts with monocytes through TLR4, and promotes in vivo bone
regeneration. Also, Magnesium (Mg) ions have been reported has immunomodulatory, reducing
LPS-induced M1 macrophage polarization. Herein, we tested the hypothesis that Fg and Mg
biomaterials, used in combination could act synergistically to modulate macrophage activation,
promoting a pro-regenerative phenotype.