Combining fibrinogen and magnesium to produce immunomodulatory biomaterials: impact on macrophages and their crosstalk with mesenchymal stem/stromal cells
AbstractMacrophage behavior upon biomaterial implantation conditions the inflammatory response, and the regenerative process.
Modulating macrophage phenotype from M1 to M2 is an increasingly explored strategy in biomaterial development. Previous work from our group, showed that Fibrinogen (Fg) interacts with monocytes through
TLR4, and promotes in vivo bone regeneration. Also, Magnesium (Mg) ions have been reported as immunomodulatory, reducing LPS-stimulated M1 macrophage polarization.
Thus, the hypothesis behind this work was that Fg and Mg biomaterials, used in combination could act synergistically to modulate macrophage activation, promoting a pro-regenerative phenotype.