Skin barrier disruptions in tape stripped and allergic dermatitis models have no effect on dermal penetration and systemic distribution of AHAPS-functionalized silica nanoparticles

Ostrowski, A.; Nordmeyer, D.; Boreham, A.; Brodwolf, R.; Mundhenk, L.; Fluhr, J.W.; Lademann, J.; Graf, C.; Ruehl, E.; Alexiev, U.; Gruber, A.D.

Journalpaper

Skin barrier disruptions in tape stripped and allergic dermatitis models have no effect on dermal penetration and systemic distribution of AHAPS-functionalized silica nanoparticles

Abstract

The skin is a potential site of entry for nanoparticles (NP) but the role of disease-associated barrier disturbances on the path and extent of skin penetration of NP remains to be characterized. Silica nanoparticles (SiO2-NP) possess promising potential for various medical applications. Here, effects of different skin barrier disruptions on the penetration of N-(6-aminohexyl)-aminopropyltrimethoxysilane (AHAPS) functionalized SiO2-NP were studied. AHAPS-SiO2-NP (55 ± 6 nm diameter)were topically applied on intact, tape stripped or on inflamed skin of SKH1 mice with induced allergic contact dermatitis for one or five consecutive days, respectively. Penetration of AHAPS-SiO2-NP through the skin was not observed regardless of the kind of barrier disruption. However, only after subcutaneous injection, AHAPS-SiO2-NP were incorporated by macrophages and transported to the regional lymph node only. Adverse effects on cells or tissues were not observed. In conclusion, AHAPS-SiO2-NP seemto not cross the normal or perturbed mouse skin.

DOI Link to Publication

URL: https://publications.hereon.de/id/32334/
Authors:Ostrowski, A., Nordmeyer, D., Boreham, A., Brodwolf, R., Mundhenk, L., Fluhr, J.W., Lademann, J., Graf, C., Ruehl, E., Alexiev, U., Gruber, A.D.
Year:2014
In: Nanomedicine: Nanotechnology, Biology and Medicine
Volume:10
Issue:7
Pages:1571 - 1581
Publisher:Elsevier, New York, NY
Type:Journalpaper
ISSN: 1549-9634
Cite as: Ostrowski, A.; Nordmeyer, D.; Boreham, A.; Brodwolf, R.; Mundhenk, L.; Fluhr, J.W.; Lademann, J.; Graf, C.; Ruehl, E.; Alexiev, U.; Gruber, A.D.: Skin barrier disruptions in tape stripped and allergic dermatitis models have no effect on dermal penetration and systemic distribution of AHAPS-functionalized silica nanoparticles. In: Nanomedicine: Nanotechnology, Biology and Medicine. Vol. 10 (2014) 7, 1571 - 1581. (DOI: 10.1016/j.nano.2014.04.004)