Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis

Muktiono, B.; Schulten, C.; Heemken, O.; Gandrass, J.; Prange, A.; Schnabl, H.; Cerboncini, C.

Journalpaper

Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis

Abstract

Protein extracts of photosystem II were prepared from leaf chloroplasts of different plant species by fast and nondenaturing methods. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot analysis of the proteins obtained showed that the extracts were enriched by D1 proteins, which appeared putatively in association with the 33-kDa oxygen-evolving-complex subunits. In further isolation steps D1 proteins were purified using salt-gradient chromatography (fast protein liquid chromatography) and characterized by western blot and mass spectrometry.

DOI Link to Publication

URL: https://publications.hereon.de/id/26578/
Authors:Muktiono, B., Schulten, C., Heemken, O., Gandrass, J., Prange, A., Schnabl, H., Cerboncini, C.
Year:2008
In: Analytical and Bioanalytical Chemistry
Volume:390
Issue:4
Pages:1195 - 1202
Publisher:Springer, Berlin / Heidelberg
Type:Journalpaper
ISSN: 1618-2642
Cite as: Muktiono, B.; Schulten, C.; Heemken, O.; Gandrass, J.; Prange, A.; Schnabl, H.; Cerboncini, C.: Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis. In: Analytical and Bioanalytical Chemistry. Vol. 390 (2008) 4, 1195 - 1202. (DOI: 10.1007/s00216-007-1781-2)