%0 journal article
%@ 2041-1723
%A Zhang, Z.,Gao, L.,Boes, A.,Bajer, B.,Stotz, J.,Apitius, L.,Jakob, F.,Schneider, E.S.,Sperling, E.,Held, M.,Emmler, T.,Schwaneberg, U.,Abetz, V.

%D 2024
%J Nature Communications
%N 
%P 3308 
%R doi:10.1038/s41467-024-47007-y
%T An Enzymatic Continuous-Flow Reactor Based on a Pore-size Matching Nano- and Isoporous Block Copolymer Membrane
%U https://doi.org/10.1038/s41467-024-47007-y
 
%X Continuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate a general-applicable and robust approach for the fabrication of a high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous block copolymer (BCP) membranes as carriers with an oriented and productive immobilization employing material binding peptides (MBP). Densely packed uniform enzyme-matched nanochannels of well-designed BCP membranes endow the desired nanoconfined environments towards a productive immobilized phytase. Tuning nanochannel properties can further regulate the complex reaction process and fortify the catalytic performance. The synergistic design of enzyme-matched carriers and efficient enzyme immobilization empowers an excellent catalytic performance with >1 month operational stability, superior productivity, and a high space-time yield (1.05 × 105 g L−1 d−1) via a single-pass continuous-flow process. The obtained performance makes the designed nano- and isoporous block copolymer membrane reactor highly attractive for industrial applications.