@misc{zhang_an_enzymatic_2024, 
author={Zhang, Z.,Gao, L.,Boes, A.,Bajer, B.,Stotz, J.,Apitius, L.,Jakob, F.,Schneider, E.S.,Sperling, E.,Held, M.,Emmler, T.,Schwaneberg, U.,Abetz, V.},
title={An Enzymatic Continuous-Flow Reactor Based on a Pore-size Matching Nano- and Isoporous Block Copolymer Membrane},
year={2024},
howpublished = {journal article},
doi = {https://doi.org/10.1038/s41467-024-47007-y},
abstract = {Continuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate a general-applicable and robust approach for the fabrication of a high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous block copolymer (BCP) membranes as carriers with an oriented and productive immobilization employing material binding peptides (MBP). Densely packed uniform enzyme-matched nanochannels of well-designed BCP membranes endow the desired nanoconfined environments towards a productive immobilized phytase. Tuning nanochannel properties can further regulate the complex reaction process and fortify the catalytic performance. The synergistic design of enzyme-matched carriers and efficient enzyme immobilization empowers an excellent catalytic performance with >1 month operational stability, superior productivity, and a high space-time yield (1.05 × 105 g L−1 d−1) via a single-pass continuous-flow process. The obtained performance makes the designed nano- and isoporous block copolymer membrane reactor highly attractive for industrial applications.},
note = {Online available at: \url{https://doi.org/10.1038/s41467-024-47007-y} (DOI). Zhang, Z.; Gao, L.; Boes, A.; Bajer, B.; Stotz, J.; Apitius, L.; Jakob, F.; Schneider, E.; Sperling, E.; Held, M.; Emmler, T.; Schwaneberg, U.; Abetz, V.: An Enzymatic Continuous-Flow Reactor Based on a Pore-size Matching Nano- and Isoporous Block Copolymer Membrane. Nature Communications. 2024. vol. 15, 3308. DOI: 10.1038/s41467-024-47007-y}}