%0 journal article
%@ 1944-8244
%A Listek, M., Hönow, A., Gossen, M., Hanack, K.

%D 2023
%J ACS Applied Materials and Interfaces
%N 37
%P 43219–43222 
%R doi:10.1021/acsami.3c05317
%T Comment on “Monoclonal Antibody Discovery based on precise selection of single transgenic hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”
%U https://doi.org/10.1021/acsami.3c05317
37 
%X In the original paper, Li and co-workers [ACS Appl. Mater. Interfaces 2022, 14, 17128–17141] described their approach to select specific hybridoma cells from a polyclonal hybridoma pool by using a cell surface anchor to catch the secreted antibody. The antigen-specific detection was performed with streptavidin-labeled antigen and a PE-labeled anti-F(ab′)2 antibody. The present comment offers a clearer description of the selection system originally published by Listek et al. in 2020 and provides further information about the importance of controls and recent adaptations made by our lab.