@misc{bosch_functional_and_2022, 
author={Bosch, C., Ackels, T., Pacureanu, A., Zhang, Y., Peddie, C.J., Berning, M., Rzepka, N., Zdora, M.-C., Whiteley, I., Storm, M., Bonnin, A., Rau, C., Margrie, T., Collinson, L., Schaefer, A.T.},
title={Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy},
year={2022},
howpublished = {journal article},
doi = {https://doi.org/10.1038/s41467-022-30199-6},
abstract = {Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues.},
note = {Online available at: \url{https://doi.org/10.1038/s41467-022-30199-6} (DOI). Bosch, C.; Ackels, T.; Pacureanu, A.; Zhang, Y.; Peddie, C.; Berning, M.; Rzepka, N.; Zdora, M.; Whiteley, I.; Storm, M.; Bonnin, A.; Rau, C.; Margrie, T.; Collinson, L.; Schaefer, A.: Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy. Nature Communications. 2022. vol. 13, no. 1, 2923. DOI: 10.1038/s41467-022-30199-6}}