@article{dorner_a_diazirinemodified_2021, 
author={Dorner, J. and Korn, P. and Gruhle, K. and Ramsbeck, D. and Garamus, V. and Lilie, H. and Meister, A. and Schwieger, C. and Ihling, C. and Sinz, A. and Drescher, S.},
title={A Diazirine-Modified Membrane Lipid to Study Peptide/Lipid Interactions – Chances and Challenges},
year={2021},
journal = {Chemistry - A European Journal},
volume = {27},
number = {59},
pages = {14586 - 14593},
doi = {10.1002/chem.202102048},
abstract = {Although incorporation of photo-activatable lipids into membranes potentially opens up novel avenues for investigating interactions with proteins, the question of whether diazirine-modified lipids are suitable for such studies, remains under debate. Focusing on the potential for studying lipid/peptide interactions by cross-linking mass spectrometry (XL-MS), we developed a diazirine-modified lipid (DiazPC), and examined its behavior in membranes incorporating the model α-helical peptide LAVA20. We observed an unexpected backfolding of the diazirine-containing stearoyl chain of the lipid. This surprising behavior challenges the potential application of DiazPC for future XL−MS studies of peptide and protein/lipid interactions. The observations made for DiazPC most likely represent a general phenomenon for any type of membrane lipids with a polar moiety incorporated into the alkyl chain. Our finding is therefore of importance for future protein/lipid interaction studies relying on modified lipid probes.},
note = {Online available at: \url{https://dx.doi.org/10.1002/chem.202102048} (DOI). Dorner, J.; Korn, P.; Gruhle, K.; Ramsbeck, D.; Garamus, V.; Lilie, H.; Meister, A.; Schwieger, C.; Ihling, C.; Sinz, A.; Drescher, S.: A Diazirine-Modified Membrane Lipid to Study Peptide/Lipid Interactions – Chances and Challenges. In: Chemistry - A European Journal. Vol. 27 (2021) 59, 14586 - 14593. (DOI:  /10.1002/chem.202102048)}}