%0 journal article
%@ 1944-8244
%A Deng, Z., Wang, W., Xu, X., Nie, Y., Liu, Y., Gould, O., Ma, N., Lendlein, A.

%D 2021
%J ACS Applied Materials and Interfaces
%N 9
%P 10748-10759 
%R doi:10.1021/acsami.0c22565
%T Biofunction of Polydopamine Coating in Stem Cell Culture
%U https://doi.org/10.1021/acsami.0c22565
9 
%X High levels of reactive oxygen species (ROS) during stem cell expansion often lead to replicative senescence. Here, a polydopamine (PDA)-coated substrate was used to scavenge extracellular ROS for mesenchymal stem cell (MSC) expansion. The PDA-coated substrate could reduce the oxidative stress and mitochondrial damage in replicative senescent MSCs. The expression of senescence-associated β-galactosidase of MSCs from three human donors (both bone marrow- and adipose tissue-derived) was suppressed on PDA. The MSCs on the PDA-coated substrate showed a lower level of interleukin 6 (IL-6), one of the senescence-associated inflammatory components. Cellular senescence-specific genes, such as p53 and p21, were downregulated on the PDA-coated substrate, while the stemness-related gene, OCT4, was upregulated. The PDA-coated substrate strongly promoted the proliferation rate of MSCs, while the stem cell character and differentiation potential were retained. Large-scale expansion of stem cells would greatly benefit from the PDA-coated substrate.