@article{deng_biofunction_of_2021, 
author={Deng, Z. and Wang, W. and Xu, X. and Nie, Y. and Liu, Y. and Gould, O. and Ma, N. and Lendlein, A.},
title={Biofunction of Polydopamine Coating in Stem Cell Culture},
year={2021},
journal = {ACS Applied Materials and Interfaces},
volume = {13},
number = {9},
pages = {10748 -10759},
doi = {10.1021/acsami.0c22565},
abstract = {High levels of reactive oxygen species (ROS) during stem cell expansion often lead to replicative senescence. Here, a polydopamine (PDA)-coated substrate was used to scavenge extracellular ROS for mesenchymal stem cell (MSC) expansion. The PDA-coated substrate could reduce the oxidative stress and mitochondrial damage in replicative senescent MSCs. The expression of senescence-associated β-galactosidase of MSCs from three human donors (both bone marrow- and adipose tissue-derived) was suppressed on PDA. The MSCs on the PDA-coated substrate showed a lower level of interleukin 6 (IL-6), one of the senescence-associated inflammatory components. Cellular senescence-specific genes, such as p53 and p21, were downregulated on the PDA-coated substrate, while the stemness-related gene, OCT4, was upregulated. The PDA-coated substrate strongly promoted the proliferation rate of MSCs, while the stem cell character and differentiation potential were retained. Large-scale expansion of stem cells would greatly benefit from the PDA-coated substrate.},
note = {Online available at: \url{https://dx.doi.org/10.1021/acsami.0c22565} (DOI). Deng, Z.; Wang, W.; Xu, X.; Nie, Y.; Liu, Y.; Gould, O.; Ma, N.; Lendlein, A.: Biofunction of Polydopamine Coating in Stem Cell Culture. In: ACS Applied Materials and Interfaces. Vol. 13 (2021) 9, 10748 -10759. (DOI:  /10.1021/acsami.0c22565)}}