@misc{listek_a_novel_2020, 
author={Listek, M., Hönow, A., Gossen, M., Hanack, K.},
title={A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line},
year={2020},
howpublished = {journal article},
doi = {https://doi.org/10.1038/s41598-020-58571-w},
abstract = {The use of monoclonal antibodies is ubiquitous in science and biomedicine but the generation and validation process of antibodies is nevertheless complicated and time-consuming. To address these issues we developed a novel selective technology based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell when they possess the desired antigen-specificity. Further the system enables the selection of desired isotypes and the screening for potential cross-reactivities in the same context. For the design of the construct we combined the transmembrane domain of the EGF-receptor with a hemagglutinin epitope and a biotin acceptor peptide and performed a transposon-mediated transfection of myeloma cell lines. The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific screening method was established. The system has been validated for globular protein antigens as well as for haptens and enables a fast and early stage selection and validation of monoclonal antibodies in one step.},
note = {Online available at: \url{https://doi.org/10.1038/s41598-020-58571-w} (DOI). Listek, M.; Hönow, A.; Gossen, M.; Hanack, K.: A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line. Scientific Reports. 2020. vol. 10, 1664. DOI: 10.1038/s41598-020-58571-w}}