%0 journal article
%@ 1743-5889
%A Miceli, E., Kuropka, B., Rosenauer, C., Osorio Blanco, E.R., Theune, L.E., Kar, M., Weise, C., Morsbach, S., freund, C., Calderon, M.

%D 2018
%J Nanomedicine
%N 20
%P 217 
%R doi:10.2217/nnm-2018-0217
%T Understanding the elusive protein corona of thermoresponsive nanogels
%U https://doi.org/10.2217/nnm-2018-0217
20 
%X Aim: We analyzed the protein corona of thermoresponsive, poly(N-isopropylacrylamide)- or poly(N-isopropylmethacrylamide)-based nanogels. Materials & methods: Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum. Results: Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly(N-isopropylacrylamide) nanogels at 37°C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation. All nanogels in their hydrophilic state are colloidally stable in serum and bear a dysopsonin-rich protein corona. Conclusion: We observed strong changes in NG stability upon slight alterations in the composition of the protein coronae according to nanogel solvation state. Nanogels in their hydrophilic state possess safe protein coronae.