@misc{volz_whitelight_supercontinuum_2018, 
author={Volz, P., Brodwolf, R., Zoschke, C., Haag, R., Schaefer-Korting, M., Alexiev, U.},
title={White-Light Supercontinuum Laser-Based Multiple Wavelength Excitation for TCSPC-FLIM of Cutaneous Nanocarrier Uptake},
year={2018},
howpublished = {journal article},
doi = {https://doi.org/10.1515/zpch-2017-1050},
abstract = {We report here on a custom-built time-correlated single photon-counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) setup with a continuously tunable white-light supercontinuum laser combined with acousto-optical tunable filters (AOTF) as an excitation source for simultaneous excitation of multiple spectrally separated fluorophores. We characterized the wavelength dependence of the white-light supercontinuum laser pulse properties and demonstrated the performance of the FLIM setup, aiming to show the experimental setup in depth together with a biomedical application. We herein summarize the physical-technical parameters as well as our approach to map the skin uptake of nanocarriers using FLIM with a resolution compared to spectroscopy. As an example, we focus on the penetration study of indocarbocyanine-labeled dendritic core-multishell nanocarriers (CMS-ICC) into reconstructed human epidermis. Unique fluorescence lifetime signatures of indocarbocyanine-labeled nanocarriers indicate nanocarrier-tissue interactions within reconstructed human epidermis, bringing FLIM close to spectroscopic analysis.},
note = {Online available at: \url{https://doi.org/10.1515/zpch-2017-1050} (DOI). Volz, P.; Brodwolf, R.; Zoschke, C.; Haag, R.; Schaefer-Korting, M.; Alexiev, U.: White-Light Supercontinuum Laser-Based Multiple Wavelength Excitation for TCSPC-FLIM of Cutaneous Nanocarrier Uptake. Zeitschrift fuer Physikalische Chemie. 2018. vol. 232, no. 5-6, 671-688. DOI: 10.1515/zpch-2017-1050}}