@misc{sarem_direct_quantification_2017, 
author={Sarem, M., Vonwil, D., Luedeke, S., Shastri, V.P.},
title={Direct quantification of dual protein adsorption dynamics in three dimensional systems in presence of cells},
year={2017},
howpublished = {journal article},
doi = {https://doi.org/10.1016/j.actbio.2017.05.021},
abstract = {The biomaterial surface upon exposure to biological fluids is covered by a layer of proteins, which is modified over a period of time and dictates the fate of the biomaterial. In this study, we present and validate a new methodology for quantification of protein adsorption on to a three-dimensional polymer scaffold from unitary and binary systems, using fluorescence molecular tomography, an optical trans-illumination technique with picomolar sensitivity. In additional to being able to follow behavior of two proteins simultaneously, this methodology is also suitable for studying protein uptake in cells situated in a polymer environment. The ability to follow protein adsorption/uptake in a continuous manner opens up new possibilities to study the role of serum proteins in biomaterial compatibility.},
note = {Online available at: \url{https://doi.org/10.1016/j.actbio.2017.05.021} (DOI). Sarem, M.; Vonwil, D.; Luedeke, S.; Shastri, V.: Direct quantification of dual protein adsorption dynamics in three dimensional systems in presence of cells. Acta Biomaterialia. 2017. vol. 57, 285-292. DOI: 10.1016/j.actbio.2017.05.021}}