%0 journal article
%@ 0142-9612
%A Shi, C., Yao, F., Li, Q., Khan, M., Ren, X., Feng, Y., Huang, J., Zhang, W.

%D 2014
%J Biomaterials
%N 25
%P 7133-7145 
%R doi:10.1016/j.biomaterials.2014.04.110
%T Regulation of the endothelialization by human vascular endothelial cells by ZNF580 gene complexed with biodegradable microparticles
%U https://doi.org/10.1016/j.biomaterials.2014.04.110
25 
%X Based on their positive charged surface, MPs can compact pEGFP-ZNF580 into MPs/pEGFP-ZNF580 complexes. The cell transfection result demonstrated that pEGFP-ZNF580 could be transported efficiently into ECs by these complexes. The result of western blot showed that the relative ZNF580 protein level can increase to 35.74%–46.11% by the overexpression of ZNF580 gene. Furthermore, the release of pEGFP-ZNF580 could be sustained at least 25 days due to the controllable degradation ability of the hydrophobic MPs' core. The MPs and MPs/pEGFP-ZNF580 complexes showed low cytotoxicity because of the introduction of PEG chains and low molecular weight PEI on the surface of these MPs. Notably, at the low concentration (20 μg/mL), the MPs and their complexes were non-cytotoxicity. The rapid endothelialization was promoted significantly by the expression of pEGFP-ZNF580.