@misc{shi_regulation_of_2014, author={Shi, C., Yao, F., Li, Q., Khan, M., Ren, X., Feng, Y., Huang, J., Zhang, W.}, title={Regulation of the endothelialization by human vascular endothelial cells by ZNF580 gene complexed with biodegradable microparticles}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.biomaterials.2014.04.110}, abstract = {Based on their positive charged surface, MPs can compact pEGFP-ZNF580 into MPs/pEGFP-ZNF580 complexes. The cell transfection result demonstrated that pEGFP-ZNF580 could be transported efficiently into ECs by these complexes. The result of western blot showed that the relative ZNF580 protein level can increase to 35.74%–46.11% by the overexpression of ZNF580 gene. Furthermore, the release of pEGFP-ZNF580 could be sustained at least 25 days due to the controllable degradation ability of the hydrophobic MPs' core. The MPs and MPs/pEGFP-ZNF580 complexes showed low cytotoxicity because of the introduction of PEG chains and low molecular weight PEI on the surface of these MPs. Notably, at the low concentration (20 μg/mL), the MPs and their complexes were non-cytotoxicity. The rapid endothelialization was promoted significantly by the expression of pEGFP-ZNF580.}, note = {Online available at: \url{https://doi.org/10.1016/j.biomaterials.2014.04.110} (DOI). Shi, C.; Yao, F.; Li, Q.; Khan, M.; Ren, X.; Feng, Y.; Huang, J.; Zhang, W.: Regulation of the endothelialization by human vascular endothelial cells by ZNF580 gene complexed with biodegradable microparticles. Biomaterials. 2014. vol. 35, no. 25, 7133-7145. DOI: 10.1016/j.biomaterials.2014.04.110}}