@misc{homaeigohar_biofunctionalized_nanofibrous_2013, author={Homaeigohar, S., Dai, T., Elbahri, M.}, title={Biofunctionalized nanofibrous membranes as super separators of protein and enzyme from water}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jcis.2013.05.076}, abstract = {Here, we report development of a novel biofunctionalized nanofibrous membrane which, despite its macroporous structure, is able to separate even trace amounts (as low as 2 mg/L) of biomolecules such as protein and enzyme from water with an optimum efficiency of ∼90%. Such an extraordinary protein selectivity at this level of pollutant concentration for a nanofibrous membrane has never been reported. In the current study, poly(acrylonitrile-co-glycidyl methacrylate) (PANGMA) electrospun nanofibers are functionalized by a bovine serum albumin (BSA) protein. This membrane is extraordinarily successful in removal of BSA protein and Candida antarctica Lipase B (Cal-B) enzyme from a water based solution. Despite a negligible non-specific adsorption of both BSA and Cal-B to the PANGMA nanofibrous membrane (8%), the separation efficiency of the biofunctionalized membrane for BSA and Cal-B reaches to 88% and 81%, respectively. The optimum separation efficiency at a trace amount of protein models is due to the water-induced conformational change of the biofunctional agent. The conformational change not only exposes more functional groups available to catch the biomolecules but also leads to swelling of the nanofibers thereby a higher steric hindrance for the solutes. Besides the optimum selectivity, the biofunctionalized membranes are highly wettable thereby highly water permeable.}, note = {Online available at: \url{https://doi.org/10.1016/j.jcis.2013.05.076} (DOI). Homaeigohar, S.; Dai, T.; Elbahri, M.: Biofunctionalized nanofibrous membranes as super separators of protein and enzyme from water. Journal of Colloid and Interface Science. 2013. vol. 406, 86-93. DOI: 10.1016/j.jcis.2013.05.076}}