%0 journal article
%@ 1618-2642
%A Proefrock, D., Leonhard, P., Ruck, W., Prange, A.

%D 2005
%J Analytical and Bioanalytical Chemistry
%N 1
%P 194-204 
%R doi:10.1007/s00216-004-2930-5
%T Development and characterisation of a new interface for coupling capillary LC with collision-cell ICP–MS and its application for phosphorylation profiling of tryptic protein digests
%U https://doi.org/10.1007/s00216-004-2930-5
1 
%X Tryptic digests of alpha and beta-casein were investigated as model compounds to demonstrate the applicability of the proposed setup for phosphorylation profiling in protein analysis using quadrupole based collision-cell ICP-MS as phosphorus specific detector. Detection limits for phosphorylated peptides down to the sub pico-mole level have been obtained. As a complementary technique, electrospray ionisation mass spectrometry (ESI-MS/MS) with data base searching has been applied to further characterise the phosphorylated peptides detected.