%0 journal article
%@ 2159-6859
%A Lau, S.,Liu, Y.,Maier, A.,Braune, S.,Gossen, M.,Neffe, A.,Lendlein, A.

%D 2021
%J MRS Communications
%N 5
%P 559-567 
%R doi:10.1557/s43579-021-00072-6
%T Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation
%U https://doi.org/10.1557/s43579-021-00072-6
5 
%X In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.