%0 journal article
%@ 2045-2322
%A Listek, M.,Hönow, A.,Gossen, M.,Hanack, K.

%D 2020
%J Scientific Reports
%N 
%P 1664 
%R doi:10.1038/s41598-020-58571-w
%T A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line
%U https://doi.org/10.1038/s41598-020-58571-w
 
%X The use of monoclonal antibodies is ubiquitous in science and biomedicine but the generation and validation process of antibodies is nevertheless complicated and time-consuming. To address these issues we developed a novel selective technology based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell when they possess the desired antigen-specificity. Further the system enables the selection of desired isotypes and the screening for potential cross-reactivities in the same context. For the design of the construct we combined the transmembrane domain of the EGF-receptor with a hemagglutinin epitope and a biotin acceptor peptide and performed a transposon-mediated transfection of myeloma cell lines. The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific screening method was established. The system has been validated for globular protein antigens as well as for haptens and enables a fast and early stage selection and validation of monoclonal antibodies in one step.