%0 journal article
%@ 1438-7492
%A Khan, M.,Yang, J.,Shi, C.,Feng, Y.,Zhang, W.,Gibney, K.,Tew, G.N.

%D 2015
%J Macromolecular Materials and Engineering
%N 8
%P 802-809 
%R doi:10.1002/mame.201500038
%T Surface Modification of Polycarbonate Urethane with Zwitterionic Polynorbornene via Thiol-ene Click-Reaction to Facilitate Cell Growth and Proliferation
%U https://doi.org/10.1002/mame.201500038
8 
%X Herein, we grafted the zwitterionic polynorbornene onto polycarbonate urethane (PCU) film surface by a convenient route of thiol-ene click-chemistry. The PCU film surface was first treated with hexamethylene-1,6-diisocynate and subsequently with two different thiol agents (l-cysteine and β-marcaptoethanol) in the presence of di-n-butyltin dilaurate (DBTDL) to immobilize sulfhydryl groups onto the surface. Here, DBTDL acted as selective catalyst for the reaction between surface-tethered isocyanates and amine/hydroxyl groups in thiol agents over that of free thiol groups. In the next step, zwitterionic polynorbornene (poly(NSulfoZI)) having functionalizable double bonds was grafted onto these surfaces by photo-initiated thiol-ene click-reaction. The modified surfaces were characterized by water contact angle and XPS analysis. Moreover, the cytocompatibility of these surfaces was investigated by model endothelial cells, EA.hy926, for 1, 3, and 7 d culture times, which showed enhanced cell adhesion and growth. Therefore, the poly(NSulfoZI) functionalized PCU surface using l-cysteine as thiol agent could be a good candidate for tissue engineering material application.