%0 journal article
%@ 1022-1352
%A Dai, T.,Miletic, N.,Loos, K.,Elbahri, M.,Abetz, V.

%D 2011
%J Macromolecular Chemistry and Physics
%N 4
%P 319-327 
%R doi:10.1002/macp.201000536
%T Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B
%U https://doi.org/10.1002/macp.201000536
4 
%X PANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to ≈50mg·g−1, and their hydrolytic activity is up to ≈2500 nmol·min−1·mg−1, much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10h heat incubation, and over 75% after 30 d storage.