%0 journal article
%@ 1582-1838
%A Ou, L.,Li, W.,Zhang, Y.,Wang, W.,Liu, J.,Sorg, H.,Furlani, D.,Gaebel, R.,Mark, P.,Klopsch, C.,Wang, L.,Luetzow, K.,Lendlein, A.,Wagner, K.,Klee, D.,Liebold, A.,Li, R.-K.,Kong, D.,Steinhoff, G.,Ma, N.

%D 2011
%J Journal of Cellular and Molecular Medicine
%N 6
%P 1310-1318 
%R doi:10.1111/j.1582-4934.2010.01086.x
%T Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model
%U https://doi.org/10.1111/j.1582-4934.2010.01086.x
6 
%X Background: Matrigel promotes angiogenesis in the myocardium from ischemic injury and prevents remodeling of the myocardium. We assessed the therapeutic efficacy of intracardiac Matrigel injection and matrigel-mediated stem cell homing in a rat myocardial infarction (MI) model.,Methods and Results: Following MI, Matrigel (250μl) or PBS was delivered by intracardiac injection. Compared to myocardial infarction control group (MI-PBS), Matrigel significantly improved left ventricular function (n=11, P<0.05) assessed by pressure-volume loops after 4 weeks. There is no significant difference in infarct size between MI-M (21.48±1.49%, n=10) and MI-PBS hearts (20.98±1.25%, n=10). The infarct wall thickness of left ventricular (LWT) is significantly higher (P<0.01) in MI-M (0.72±0.02mm, n=10) compared with MI-PBS (0.62±0.02mm, n=10). MI-Matrigel (MI-M) hearts exhibited higher capillary density (border 130.8±4.7 vs.115.4 ± 6.0, P<0.05; vessels per HPF (400×), n=6) than MI-PBS hearts. c-Kit+ stem cells (38.3±5.3 vs. 25.7±1.5 c-Kit+ cells per HPF(630×), n=5, P<0.05) and CD34+ cells (13.0±1.51 vs. 5.6±0.68 CD34+ cells per HPF (630×), n=5, P<0.01) were significantly more numerous in MI-Matrigel than in MI-PBS in the infarcted hearts (n=5, P<0.05).,Conclusions: Intracardiac Matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34+ and c-Kit+ stem cells.