%0 journal article
%@ 1022-1336
%A Miletic, N.,Abetz, V.,Ebert, K.,Loos, K.

%D 2010
%J Macromolecular Rapid Communications
%N 1
%P 71-74 
%R doi:10.1002/marc.200900497
%T Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles
%U https://doi.org/10.1002/marc.200900497
1 
%X Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.