%0 journal article
%@ 1567-5769
%A Butschak, G.,Karsten, U.,Schelhaas, U.,Ott, H.,Emmendoerffer, A.,Niemeyer, B.,Helmholz, H.,Goletz, S.

%D 2006
%J International Immunopharmacology
%N 9
%P 1441-1450 
%R doi:10.1016/j.intimp.2006.04.010
%T Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococus fascians
%U https://doi.org/10.1016/j.intimp.2006.04.010
9 
%X In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic,bacterium, Rhodococcus fascians, were able to induce cytokine release (TNFα) from mouse peritoneal macrophages. Monoclonal,antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high,molecular (MWN100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA,variants for its determination were developed.