%0 journal article
%@ 0168-1656
%A Heilmann, K.,Groth, T.,Bersing, O.,Albrecht, W.,Schossig, M.,Lendlein, A.,Micheel, B.

%D 2005
%J Journal of Biotechnology
%N 3
%P 291-301 
%R doi:10.1016/j.jbiotec.2004.09.004
%T The Influence of Chemical Composition of Cell Culture Material on Growth and Antibody Production of Hybridoma Cells
%U https://doi.org/10.1016/j.jbiotec.2004.09.004
3 
%X The multiplication and antibody production of murine hybridoma cells cultured on five different polymer membranes were tested and compared with conventional tissue culture polystyrene (TCPS). Membranes were prepared from  polyacrylonitrile (PAN) and acrylonitrile copolymerized with N-vinylpyrrolidone (NVP20, NVP30), Na-methallylsulfonat (NaMAS) and N-(3-amino-propyl-methacrylamide-hydrochloride) (APMA). Cell number and antibody concentration were quantified as criteria for viability and productivity. Adhesion of hybridoma cells was characterized by vital and scanning electron microscopy. The results suggest that a strong adhesion of cells, observed on APMA and TCPS, increased cell  growth but reduced monoclonal antibody production. In contrast membranes with lowered adhesivity such as NVP20 provided favourable conditions for monoclonal antibody production. In addition it was shown that this membrane possessed also a minor fouling as indicated by the low decrease of water flux across the membrane after protein adsorption. It was concluded that NVP20 could be a suitable material for the development of hollow fibre membranes for bioreactors.