@misc{roch_urinederived_renal_2025, 
author={Roch, Toralf,Paniskaki, Krystallenia,Wehler, Patrizia,Thieme, Constantin J.,Moradian, Hanieh,Blazquez-Navarro, Arturo,Anft, Moritz,Reinke, Petra,Westhoff, Timm H.,Stervbo, Ulrik,Babel, Nina},
title={Urine-derived renal tubular epithelial cells resemble functional characteristics of professional antigen-presenting cells and can directly induce BKV-specific T cell responses},
year={2025},
howpublished = {journal article},
doi = {https://doi.org/10.1007/s40620-025-02298-2},
abstract = {Background: Reactivation of the BK virus (BKV) is a critical adverse event after kidney transplantation and can lead to graft loss. BKV-reactive T cell-mediated viral control can be facilitated by reducing immunosuppression. However, the exact mechanism underlying the T cell-mediated BKV clearance in the kidney transplant is not clear.
Methods: Here, we used urine-derived renal tubular epithelial cells as a model system to investigate the immunomodulatory capacity of urine-derived renal tubular epithelial cells and their potential to induce T cell responses against BKV. Urinederived renal tubular epithelial cells were generated by culturing urine-derived cell pellets. To assess the inflammatory potential of urine-derived renal tubular epithelial cells, the cells were treated with Poly I:C or TNFα/IFNγ. To investigate urine-derived renal tubular epithelial cell-induced T cell responses, autologous T cells, isolated from blood were co-cultured with urine-derived renal tubular epithelial cells, in the presence of BKV protein-derived peptides and PolyI:C or TNFα/ IFNγ. BKV-reactive T cells,  ytokine/chemokine secretion and expression of co-stimulatory molecules were evaluated using multiplex  ssays and multi-parameter flow cytometry.},
note = {Online available at: \url{https://doi.org/10.1007/s40620-025-02298-2} (DOI). Roch, T.; Paniskaki, K.; Wehler, P.; Thieme, C.; Moradian, H.; Blazquez-Navarro, A.; Anft, M.; Reinke, P.; Westhoff, T.; Stervbo, U.; Babel, N.: Urine-derived renal tubular epithelial cells resemble functional characteristics of professional antigen-presenting cells and can directly induce BKV-specific T cell responses. Journal of Nephrology. 2025. DOI: 10.1007/s40620-025-02298-2}}