@misc{zhang_rapid_protein_2025, 
author={Zhang, Zhenzhen,Rahman, Md. Mushfequr,Bajer, Barbara,Abetz, Volker},
title={Rapid protein fractionation with tunable amphiphilic isoporous block copolymer membranes},
year={2025},
howpublished = {journal article},
doi = {https://doi.org/10.1016/j.memsci.2025.124114},
abstract = {Isoporous block copolymer membranes have emerged as a promising platform for high-performance protein separation. However, it remains a significant challenge to achieve a robust protein fractionation system with both high selectivity and permeance. Herein, we established the tunable isoporous membranes derived from the amphiphilic polystyrene-block-poly(2-hydroxyethyl methacrylate) (PS-b-PHEMA) block copolymer. The membrane characteristics, i.e., pore size, surface porosity, hydrophilicity, and surface charge, were tuned by varying the PS-b-PHEMA molecular weight and composition. The protein separation performance of the designed tunable membranes was thoroughly investigated under application-relevant cross-flow mode by varying the cross-flow rate. Different mixed protein systems, including bovine serum albumin (BSA) and lysozyme (LZ), β-lactoglobulin (LG) and BSA, hemoglobin (HB) and BSA, were tested in a readily accessible and stable physiological buffer at pH = 7.4. The separation behavior is governed by the synergistic effects of size sieving, electrostatic interactions, and competition among the permeating proteins. The prepared membranes achieved an unprecedented selectivity of 88.8 and a high permeance of 67 L m−2 h−1 bar−1 for the LZ/BSA protein mixture, overcoming the conventional trade-off between permeance and selectivity.},
note = {Online available at: \url{https://doi.org/10.1016/j.memsci.2025.124114} (DOI). Zhang, Z.; Rahman, M.; Bajer, B.; Abetz, V.: Rapid protein fractionation with tunable amphiphilic isoporous block copolymer membranes. Journal of Membrane Science. 2025. vol. 727, 124114. DOI: 10.1016/j.memsci.2025.124114}}