@misc{listek_comment_on_2023, author={Listek, M.,Hönow, A.,Gossen, M.,Hanack, K.}, title={Comment on “Monoclonal Antibody Discovery based on precise selection of single transgenic hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”}, year={2023}, howpublished = {journal article}, doi = {https://doi.org/10.1021/acsami.3c05317}, abstract = {In the original paper, Li and co-workers [ACS Appl. Mater. Interfaces 2022, 14, 17128–17141] described their approach to select specific hybridoma cells from a polyclonal hybridoma pool by using a cell surface anchor to catch the secreted antibody. The antigen-specific detection was performed with streptavidin-labeled antigen and a PE-labeled anti-F(ab′)2 antibody. The present comment offers a clearer description of the selection system originally published by Listek et al. in 2020 and provides further information about the importance of controls and recent adaptations made by our lab.}, note = {Online available at: \url{https://doi.org/10.1021/acsami.3c05317} (DOI). Listek, M.; Hönow, A.; Gossen, M.; Hanack, K.: Comment on “Monoclonal Antibody Discovery based on precise selection of single transgenic hybridomas with an On-Cell-Surface and Antigen-Specific Anchor”. ACS Applied Materials and Interfaces. 2023. vol. 15, no. 37, 43219–43222. DOI: 10.1021/acsami.3c05317}}