@misc{dorner_a_diazirinemodified_2021, 
author={Dorner, J.,Korn, P.,Gruhle, K.,Ramsbeck, D.,Garamus, V.,Lilie, H.,Meister, A.,Schwieger, C.,Ihling, C.,Sinz, A.,Drescher, S.},
title={A Diazirine-Modified Membrane Lipid to Study Peptide/Lipid Interactions – Chances and Challenges},
year={2021},
howpublished = {journal article},
doi = {https://doi.org/10.1002/chem.202102048},
abstract = {Although incorporation of photo-activatable lipids into membranes potentially opens up novel avenues for investigating interactions with proteins, the question of whether diazirine-modified lipids are suitable for such studies, remains under debate. Focusing on the potential for studying lipid/peptide interactions by cross-linking mass spectrometry (XL-MS), we developed a diazirine-modified lipid (DiazPC), and examined its behavior in membranes incorporating the model α-helical peptide LAVA20. We observed an unexpected backfolding of the diazirine-containing stearoyl chain of the lipid. This surprising behavior challenges the potential application of DiazPC for future XL−MS studies of peptide and protein/lipid interactions. The observations made for DiazPC most likely represent a general phenomenon for any type of membrane lipids with a polar moiety incorporated into the alkyl chain. Our finding is therefore of importance for future protein/lipid interaction studies relying on modified lipid probes.},
note = {Online available at: \url{https://doi.org/10.1002/chem.202102048} (DOI). Dorner, J.; Korn, P.; Gruhle, K.; Ramsbeck, D.; Garamus, V.; Lilie, H.; Meister, A.; Schwieger, C.; Ihling, C.; Sinz, A.; Drescher, S.: A Diazirine-Modified Membrane Lipid to Study Peptide/Lipid Interactions – Chances and Challenges. Chemistry - A European Journal. 2021. vol. 27, no. 59, 14586-14593. DOI: 10.1002/chem.202102048}}