@misc{ridolfi_gold_nanoparticles_2020, 
author={Ridolfi, A.,Caselli, L.,Montis, C.,Mangiapia, G.,Berti, D.,Brucale, M.,Valle, F.},
title={Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation},
year={2020},
howpublished = {journal article},
doi = {https://doi.org/10.1111/jmi.12910},
abstract = {Inorganic nanoparticles (NPs) represent promising examples of engineered nanomaterials, providing interesting biomedical solutions in several fields, like therapeutics and diagnostics. Despite the extensive number of investigations motivated by their remarkable potential for nanomedicinal applications, the interactions of NPs with biological interfaces are still poorly understood. The effect of NPs on living organisms is mediated by biological barriers, such as the cell plasma membrane, whose lateral heterogeneity is thought to play a prominent role in NPs adsorption and uptake pathways. In particular, biological membranes feature the presence of rafts, that is segregated lipid micro and/or nanodomains in the so‐called liquid ordered phase (Lo), immiscible with the surrounding liquid disordered phase (Ld). Rafts are involved in various biological functions and act as sites for the selective adsorption of materials on the membrane. Indeed, the thickness mismatch present along their boundaries generates energetically favourable conditions for the adsorption of NPs. Despite its clear implications in NPs internalisation processes and cytotoxicity, a direct proof of the selective adsorption of NPs along the rafts’ boundaries is still missing to date. Here we use multicomponent supported lipid bilayers (SLBs) as reliable synthetic models, reproducing the nanometric lateral heterogeneity of cell membranes. After being characterised by atomic force microscopy (AFM) and neutron reflectivity (NR), multidomain SLBs are challenged by prototypical inorganic nanoparticles, that is citrated gold nanoparticles (AuNPs), under simplified and highly controlled conditions. By exploiting AFM, we demonstrate that AuNPs preferentially target lipid phase boundaries as adsorption sites. The herein reported study consolidates and extends the fundamental knowledge on NPs–membrane interactions, which constitute a key aspect to consider when designing NPs‐related biomedical applications.},
note = {Online available at: \url{https://doi.org/10.1111/jmi.12910} (DOI). Ridolfi, A.; Caselli, L.; Montis, C.; Mangiapia, G.; Berti, D.; Brucale, M.; Valle, F.: Gold nanoparticles interacting with synthetic lipid rafts: an AFM investigation. Journal of Microscopy. 2020. vol. 280, no. 3, 194-203. DOI: 10.1111/jmi.12910}}