@misc{klopsch_spray_and_2015, 
author={Klopsch, C.,Gaebel, R.,Kaminski, A.,Mark, P.,Wang, W.,Toelk, A.,Delyagina, E.,Kleiner, G.,Koch, L.,Chichkov, B.,Mela, P.,Jockenhoevel, S.,Ma, N.,Steinhoff, G.},
title={Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering},
year={2015},
howpublished = {journal article},
doi = {https://doi.org/10.1002/term.1657},
abstract = {At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced,and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies,,spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue,engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs),,umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on,poly(«-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in,stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun,PCL tissue equivalents. Graftsweremonitored in vitro under static and dynamic conditions in bioreactors.,SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern,,respectively. The engineered valves demonstrated immediate sufficient performance, complete cell,coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular,matrix deposition. SaBP revealed higher efficiency,with at least 12-fold shorter processing time than the,applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell–scaffold distance.,Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in,complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP,might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns.},
note = {Online available at: \url{https://doi.org/10.1002/term.1657} (DOI). Klopsch, C.; Gaebel, R.; Kaminski, A.; Mark, P.; Wang, W.; Toelk, A.; Delyagina, E.; Kleiner, G.; Koch, L.; Chichkov, B.; Mela, P.; Jockenhoevel, S.; Ma, N.; Steinhoff, G.: Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering. Journal of Tissue Engineering and Regenerative Medicine. 2015. vol. 9, no. 12, E177-E190. DOI: 10.1002/term.1657}}