@misc{kohl_nearinfrared_dyeloaded_2011, 
author={Kohl, Y.,Kaiser, C.,Bost, W.,Stracke, F.,Thielecke, H.,Wischke, C.,Lendlein, A.,Kratz, K.,Lemor, R.},
title={Near-infrared dye-loaded PLGA nanoparticles prepared by spray drying for photoacoustic applications},
year={2011},
howpublished = {journal article},
doi = {https://doi.org/10.5301/IJAO.2011.6405},
abstract = {Introduction: Nanoparticulate contrast agents are of great interest for diagnostic applications with high resolution in medicine. Here we present polymer-based degradable nanoparticles exhibiting a near infrared (NIR) absorption suitable for photoacoustic imaging.,Methods: The nanoparticles were prepared by incorporation of indocyanine green (ICG) as NIR dye in poly[(rac-lactide)-co-glycolide] (PLGA) via an optimized spray drying process. By application of a multi-step centrifugation protocol, two different size fractions were achieved. The biocompatibitilty of the nanoparticles was tested in 2D cell cultures (human hepatocarcinoma cells and monkey kidney cells) using WST-1, BrdU and LDH assay.,Results: Spherical particles were obtained with a good yield (>81%), showing a high NIR-dye encapsulation efficiency (>98%). By multi-step centrifugation, two different size fractions with a mean diameter of 640 nm and 390 nm were obtained. Cytotoxicity studies of the synthesized ICG-loaded PLGA particles were performed. No cytotoxic effect on metabolic activity, proliferation, or membrane integrity was observed.,Conclusion: The high optical absorption at the relevant NIR-wavelength around 800 nm in combination with absence of cytotoxicity qualifies the ICG-loaded PLGA particles as promising candidates for degradable photoacoustic contrast agents.},
note = {Online available at: \url{https://doi.org/10.5301/IJAO.2011.6405} (DOI). Kohl, Y.; Kaiser, C.; Bost, W.; Stracke, F.; Thielecke, H.; Wischke, C.; Lendlein, A.; Kratz, K.; Lemor, R.: Near-infrared dye-loaded PLGA nanoparticles prepared by spray drying for photoacoustic applications. The International Journal of Artificial Organs. 2011. vol. 34, no. 2, 249-252. DOI: 10.5301/IJAO.2011.6405}}