@misc{wang_localized_sdf1alpha_2010, 
author={Wang, W.,Li, W.,Ong, L.-L.,Furlani, D.,Kaminski, A.,Liebold, A.,Luetzow, K.,Lendlein, A.,Wang, J.,Li, R.-K.,Steinhoff, G.,Ma, N.},
title={Localized SDF-1alpha gene release mediated by collagen substrate induces CD117+ stem cell homing},
year={2010},
howpublished = {journal article},
doi = {https://doi.org/10.1111/j.1582-4934.2008.00624.x},
abstract = {Objective: Stromal cell-derived factor-1α (SDF-1α) mediated mobilization and homing of stem cells showed promising potential in stem cell based tissue engineering and regenerative medicine. However local and sustained release of SDF-1α is indispensable for stem cell mediated regenerative process due to its short half-life under inflammatory conditions. In this study, a gene activated collagen substrate (GAC) was formed via assembly of plasmid encoding SDF-1α into a collagen substrate to create a microenvironment favoring stem cell homing. Local release of SDF-1α from the transfected cells on GAC and its effect on CD117+ stem cell homing were investigated in a mouse hindlimb model.,Methods: Non-viral poly-ethyleneimine (25kDa PEI)/DNA complexes were mixed with rat tail collagen solution to form the GAC. Optimization of GAC was carried out based on collagen effects on the PEI/DNA complexes, viability and luciferase expression of COS7 cells on GAC. CD117+ stem cells homing in response to SDF-1α local expression from transfected cells on GAC were investigated in a flow chamber in vitro and in a mouse hindlimb model in vivo. The gene expression, migration of CD117+ stem cells and the induced inflammation were investigated with immunostaining, reverse transcription polymerase chain reaction (RT-PCR) and H&E staining.,Results: The optimized parameters for GAC were DNA dosage 10μg/cm2, molar ratio of PEI nitrogen in primary amine to DNA phosphate (N/P ratio) 4 and mass ratio of collagen to DNA (C/D ratio) 1.0. It kept cell viability above 75% and transfection efficiency around 5.8 × 105 RLU/mg protein. GAC allowed the sustained gene release up to 60 days. GAC mediated SDF-1α gene release induced migration and homing of CD117+ stem cells in vitro and in vivo significantly, and the inflammation of GAC reduced significantly two weeks after transplantation. GAC is a promising stem cell based therapeutic strategy for regenerative medicine.},
note = {Online available at: \url{https://doi.org/10.1111/j.1582-4934.2008.00624.x} (DOI). Wang, W.; Li, W.; Ong, L.; Furlani, D.; Kaminski, A.; Liebold, A.; Luetzow, K.; Lendlein, A.; Wang, J.; Li, R.; Steinhoff, G.; Ma, N.: Localized SDF-1alpha gene release mediated by collagen substrate induces CD117+ stem cell homing. Journal of Cellular and Molecular Medicine. 2010. vol. 14, no. 1-2, 392-402. DOI: 10.1111/j.1582-4934.2008.00624.x}}