@misc{boese_membranes_from_2007, 
author={Boese, G.,Trimpert, C.,Albrecht, W.,Malsch, G.,Groth, T.,Lendlein, A.},
title={Membranes from Acrylonitrile-Based Polymers for Selective Cultivation of Human Keratinocytes},
year={2007},
howpublished = {journal article},
doi = {https://doi.org/10.1089/ten.2006.0442},
abstract = {A cell carrier made from synthetic material supporting selective growth of keratinocytes is a promising approach to avoid the phenomenon of fibroblast overgrowth during in vitro culture of skin substitutes.,Therefore, we investigated polymer membranes made of polyacrylonitrile and copolymers of acrylonitrile,and N-vinylpyrrolidone (NVP) for their ability to support selectively the growth of keratinocytes. It was,found that a copolymer with an NVP-content of 30% (NVP30) supports growth of human keratinocyte cell line (HaCaT) cells and inhibits fibroblast growth under serum-containing conditions. Cell proliferation of HaCaT cells was measured over 14 days. If both cell types were cultured under serum-free conditions for initial adhesion over 6 h on these NVP30 polymers, they adhered to the same extent. Long-term experiments over 7 days were performed as a coculture of both cell types showing that HaCaT cells had a growth advantage that seems to be related to the paracrine activity of contaminating fibroblasts. As a result, confluent layers of HaCaT cells were obtained with small numbers of remaining fibroblasts. The new poly,[acrylonitrile-co(NVP) membranes seem to be a promising culture system for the production of epidermal,transplants.},
note = {Online available at: \url{https://doi.org/10.1089/ten.2006.0442} (DOI). Boese, G.; Trimpert, C.; Albrecht, W.; Malsch, G.; Groth, T.; Lendlein, A.: Membranes from Acrylonitrile-Based Polymers for Selective Cultivation of Human Keratinocytes. Tissue Engineering. 2007. vol. 13, no. 12, 2995-3302. DOI: 10.1089/ten.2006.0442}}