@misc{siwek_isolation_of_2005, 
author={Siwek, M.,Galunsky, B.,Niemeyer, B.},
title={Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis},
year={2005},
howpublished = {journal article},
doi = {https://doi.org/10.1007/s00216-004-2936-z},
abstract = {Total selenium content and the selenium distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction and centrifugation of Antarctic krill samples were determined. About 26% of the total selenium ( Se) (2.4 μg/g dry weight) was found in the supernatant and the rest was in the pellet. The digestion of the protein and the isolation of low molecular selenium-containing fractions was achieved by enzymatic hydrolysis followed by size-exclusion chromatography in conjunction with graphite furnace atomic absorption spectrometry. From the applied various proteinases (trypsin, -chymotrypsin, subtilisin, pronase E), the treatment with pronase E led to best recovery of the total Se. About 96% of the total selenium was found in the hydrolysate, mainly in low molecular weight fractions. 15% of the low molecular weight Se-species were in fractions with molecular weights in the range of amino acids.},
note = {Online available at: \url{https://doi.org/10.1007/s00216-004-2936-z} (DOI). Siwek, M.; Galunsky, B.; Niemeyer, B.: Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis. Analytical and Bioanalytical Chemistry. 2005. vol. 381, no. 3, 737-741. DOI: 10.1007/s00216-004-2936-z}}