@misc{klopsch_intracardiac_injection_2009, 
author={Klopsch, C., Furlani, D., Gaebel, R., Li, W., Pittermann, E., Ugurlucan, M., Kundt, G., Zingler, C., Titze, U., Wang, W., Ong, L.-L., Wagner, K., Li, R.-K., Ma, N., Steinhoff, G.},
title={Intracardiac injection of erythropoietin induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model},
year={2009},
howpublished = {journal article},
doi = {https://doi.org/10.1111/j.1582-4934.2008.00546.x},
abstract = {Erythropoietin (EPO) protects the myocardium from ischaemic injury and promotes beneficial remodelling. We assessed the therapeutic efficacy of intracardiac EPO injection and EPO-mediated stem cell homing in a rat myocardial infarction (MI) model. Following MI, EPO (3000 U/kg) or saline was delivered by intracardiac injection. Compared to myocardial infarction control group (MIC), EPO significantly improved left ventricular function (n= 11–14, P< 0.05) and decreased right ventricular wall stress (n= 8, P< 0.05) assessed by pressure-volume loops after 6 weeks. MI-EPO hearts exhibited smaller infarction size (20.1 ± 1.1%versus 27.8 ± 1.2%; n= 6–8, P< 0.001) and greater capillary density (338.5 ± 14.7 versus 259.8 ± 9.2 vessels per mm; n= 6–8, P< 0.001) than MIC hearts. Direct EPO injection reduced post-MI myocardial apoptosis by approximately 41% (0.27 ± 0.03%versus 0.42 ± 0.03%; n= 6, P= 0.005). The chemoattractant SDF-1 was up-regulated significantly assessed by quantitative realtime PCR and immunohistology. c-Kit+ and CD34+ stem cells were significantly more numerous in MI-EPO than in MIC at 24 hrs in peripheral blood (n= 7, P< 0.05) and 48 hrs in the infarcted hearts (n= 6, P< 0.001). Further, the mRNAs of Akt, eNOS and EPO receptor were significantly enhanced in MI-EPO hearts (n= 7, P< 0.05). Intracardiac EPO injection restores myocardial functions following MI, which may attribute to the improved early recruitment of c-Kit+ and CD34+ stem cells via the enhanced expression of chemoattractant SDF-1.},
note = {Online available at: \url{https://doi.org/10.1111/j.1582-4934.2008.00546.x} (DOI). Klopsch, C.; Furlani, D.; Gaebel, R.; Li, W.; Pittermann, E.; Ugurlucan, M.; Kundt, G.; Zingler, C.; Titze, U.; Wang, W.; Ong, L.; Wagner, K.; Li, R.; Ma, N.; Steinhoff, G.: Intracardiac injection of erythropoietin induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model. Journal of Cellular and Molecular Medicine. 2009. vol. 13, no. 4, 664-679. DOI: 10.1111/j.1582-4934.2008.00546.x}}